Please use this identifier to cite or link to this item: http://library.meduniv.lviv.ua/jspui/handle/123456789/378
Title: Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients
Authors: Zasońska, Beata A.
Hlídková, Helena
Petrovský, Eduard
Myronovskij, Severyn
Nehrych, Tetyana
Negrych, Nazar
Shorobura, Mariya
Antonyuk, Volodymyr
Stoika, Rostyslav
Kit, Yuriy
Horák, Daniel
Keywords: Magnetic microspheres
Functionalization
Affinity chromatography
p46/Myo1C protein
Autoantibody
Immunoglobulin M
Autoimmune disease marker
Multiple sclerosis
Issue Date: 2018
Abstract: Monodisperse nonmagnetic macroporous poly(glycidyl methacrylate) (PGMA) microspheres were synthesized by multistep swelling polymerization of glycidyl methacrylate, ethylene dimethacrylate and 2-[(methoxycarbonyl)methoxy]ethyl methacry-late (MCMEMA). This was followed (a) by ammonolysis to modify the microspheres with amino groups, and (b) by incorporation of iron oxide (γ-Fe2O3) into the pores to render the particles magnetic. The resulting porous and magnetic microspheres were characterized by scanning and transmission electron microscopy (SEM and TEM), atomic absorption and Fourier transform infrared spectroscopy (AAS and FTIR), elemental analysis, vibrating magnetometry, mercury porosimetry and Brunauer-Emmett-Teller adsorption/desorption isotherms. The microspheres are meso- and macroporous, typically 5 μmindiameter, contain 0.9 mM g−1 of amino groups and 14 wt.% of iron according to elemental analysis and AAS, respectively. The particles were conjugated to p46/Myo1C protein, a potential biomarker of autoimmune diseases, to isolate specific autoantibodies in the blood of pati ents suffering from multiple sclerosis (MS). The p4 6/Myo 1C loaded mic rosphe res are shown to enable the preconcentration of minute quantities of specific immunoglobulins prior to their quantification via SDS-PAGE. The immunoglobulin M (IgM) with affinity to Myo1C was detected in MS patients.
URI: http://194.44.178.67/jspui/handle/123456789/378
Appears in Collections:КН ⟶ Наукові праці

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Monodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with aff inity for the 46 kDa form of unconventional Myo1C present in autoimmune patients.pdfMonodisperse magnetic poly(glycidyl methacrylate) microspheres for isolation of autoantibodies with affinity for the 46 kDa form of unconventional Myo1C present in autoimmune patients / Beata A. Zasońska, Helena Hlídková, Eduard Petrovský, Severyn Myronovskij, Tetyana Nehrych, Nazar Negrych, Mariya Shorobura, Volodymyr Antonyuk, Rostyslav Stoika, Yuriy Kit, Daniel Horák // Microchimica Acta. – 2018.1.81 MBAdobe PDFView/Open


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