Please use this identifier to cite or link to this item: http://library.meduniv.lviv.ua/jspui/handle/123456789/376
Title: Identification of the Unique Properties of IgGs and their Heavy chains in Blood Serum of Multiple Sclerosis Patients
Authors: Negrych, Nazar
Myronovskij, Severyn
Nehrych, Tetyana
Kit, Yuriy
Keywords: IgGs
Heavy chains
Blood serum
Multiple sclerosis
Issue Date: 2016
Abstract: Monitoring of multiple sclerosis (MS) requires of molecular markers. Recently, using TCA-precipitation/ extraction and MALDI TOF/TOF mass-spectrometry, we identified in blood serum of multiple sclerosis (MS) patients earlier unknown 46 kDa form of human unconventional myosin IC isoform b (Myo1C) (Myronovskij et al., 2015). During SDS-electrophoresis of TCA-extracted proteins of blood serum of 28 MS patients, 3 additional polypeptides with 55, 50 and 25 kDa molecular masses were detected. Western-blot analysis using monospecific anti-human IgG rabbit HRP-conjugated antibodies showed that 55 and 25 kDa polypeptides belong to heavy and light chains of IgG molecules, while 50 kDa protein corresponds to free heavy chains of IgGs. None of these polypeptides was found in fractions of TCA- extracted polypeptides isolated from blood serum of healthy human donors or patients with systemic lupus erythematosis and rheumatoid arthritis.
URI: http://194.44.178.67/jspui/handle/123456789/376
ISSN: 2471-8513
Appears in Collections:КН ⟶ Наукові праці

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Identification-of-the-unique-properties-of-iggs-and-their-heavy-chains-in-bloodserum-of-multiple-sclerosis-patients.pdfIdentification of the Unique Properties of IgGs and their Heavy chains in Blood Serum of Multiple Sclerosis Patients / Nazar Negrych, Severyn Myronovskij, Tetyana Nehrych, Rostyslav Stoika, Yuriy Kit // Journal of Autoimmune Disorders. – 2016. – Vol.2. – P. 1–5.721.82 kBAdobe PDFView/Open


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